P43 Impact of slow-freezing-thawing on the gene expression profile of human metaphase II oocytes: transcriptomic study
نویسندگان
چکیده
منابع مشابه
Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.
BACKGROUND Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes. METHO...
متن کاملPolscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
BACKGROUND The clinical efficacy of the current methods used for cryopreservation of metaphase II human oocytes is low. Meiotic spindle disorders are thought to be largely responsible for this situation. METHODS Supernumerary fresh metaphase II human oocytes were cryopreserved in 1,2-propanediol with 0.1 M sucrose using a slow freezing/rapid thawing programme. Meiotic spindles were analysed i...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2010
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)62359-0